1. Fluorescence meansSpontaneous illumination in darkRelease of longer wavelength light on absorbing light of shorter wavelengthRelease of shorter wavelength light on absorbing light of longer wavelengthRelease of equal wavelength light at constant rate 2. The genetic defect in Refsum's disease lies inAlpha oxidation of fatty acidsLDL receptorsRecognition of Apo A1Fatty acid synthase complex 3. Leucine zipper complex isDNA binding proteinMembrane attack complexB cell epitomesReceptor ligand protein 4. Apo E, Apo C is synthesised byLiverkidneyIntestineRBCs 5. Ketone bodies cannot be utilised byHeartBrainSkeletal musclesRBCs 6. Dementia, dermatitis, diarrhoea are features ofBeri beriPellagraVitamin B12 deficiencyPyridoxine deficiency 7. Increased pyruvate and lactate are found in deficiency ofThiamineNiacinVitamin CPyridoxine 8. Which vitamin is necessary for coenzyme A synthesispantothenic acidAscorbic acidBiotinpyridoxine 9. Gout is a disorder ofPurine metabolismPyrimidine metabolismOxalate metabolismProtein metabolism 10. Ubiquitin is involved inIntracellular proteolysisElectron transport chainTransport of ATPProtein folding 11. Which of the following is considered the active form of calciumIonised calciumAlbumin bound calciumPhosphate bound calciumProtein bound Calcium 12. Western blot technique is done forMitochondrial RNADouble strand DNAr-RNAProtein 13. Vitamin K is involved in post translation modification ofGlutamateAspartateLysineProline 14. Which amino acid will convert to pyruvateAlanineArginineHistidineProline 15. Rate limting step in fatty acid synthesis isProduction of acetyl CoAProduction of oxaloacetateProduction of malonyl CoAProduction of citrate 16. Copper containing enzyme in collagen synthesisLysyl hydroxylaseLysyl oxidaseProcollagen hydroxylaseProcollagen peptidase 17. RNA polymerase does not requireTemplate(ds DNA)Activated precursors(ATPDivalent metal ions(Mn2+,Mg2+)Primer 18. A common feature of all serine proteases isAutocatalytic activation of zymogen precursorTight binding of pancreatic trypsin inhibitorPresence of Ser-His-Asp catalytic triad at the active siteCleavage of protein on the carboxyl site of serine residues 19. Triplex DNA is due toHoogsteen pairingPalindromic sequencesPolypyramidine tractsLarge number of guanosine repeats 20. Poly(A) tail on translation will give rise toPolyglycinePolylysinePolyalaninePolyproline 21. in cytochrome P450,P stands forstructural proteinsubstrate proteinpigmentpolymer 22. An enzyme that makes a double stranded DNA copy from a single stranded RNA template molecule is known asDNA polymerase IRNA polymeraseReverse transcriptasePhosphokinase 23. Biotin is required for the activity ofPyruvate carboxylaseLactate dehydrogenaseSuccinate thiokinasePhosphohexose isomerase 24. Most commonly used vector for DNA cloningPlasmidVirusCosmidPhage 25. Cori disease occurs due to deficiency ofGlucose 6 phosphataseHexokinaseDebranching enzymePFK 26. Beta oxidation of very long chain fatty acids occursCytoplasmEndoplasmic reticulumLysosomePeroxisomes 27. Most important tool used in genetic engineeringGenesEnzymesPeptidyl-transferaseRibozymes 28. Enzyme involved in initiation of peptide chain synthesisTopoisomeraseTransformylaseRNA polymerasePeptidyl transferase 29. Identification of individual by their DNA was invented byShapiroLewisJeffreysPasture 30. true about ribozymes iscut DNA at specific siteGTPase activitypeptidyl transferase activityparticipate in DNA synthesis 31. Thermostability in DNA is contributed mostly byA=TG=CMolecular baseParallel arrangement 32. Deficiency of which vitamin causes lactic acid acidosisRiboflavinThiamineNiacinPantothenic acid 33. Increased level of lipoprotein (a) predisposes toLiver cirrhosisAtherosclerosisNephrotic syndromePancreatitis 34. Which type of lipase is controlled by glucagonHormone sensitive lipaseLipoprotein lipaseGastric lipasePancreatic lipase 35. The end product of cytosol fatty acid synthetase in humans isOleic acidArachidonic acidLinoleic acidPalmitic acid 36. Which method is used to seperate a mixture of lipidsElectrophoresisChromatographyIsoelectric focusingPAGE 37. enzyme involved in degradation of epinephrinecholinesteraseanticholinesterasecatechol O-transferaseoxidases 38. In liver, ethanol is converted toMethyl alcoholPyruvateAcetaldehydeOxaloacetate 39. Mitochondrial DNA isClosed circularNicked circularLinearOpen Circular 40. In the mammalian genome, maximum number of genes code for the receptors ofGrowth factorsImmunoglobulin receptorsOdorantsInterleukins 41. Fatty acid elongation occurs in which part of cellCytosolMitochondriaLysosomeNucleus 42. Steroidal hormones are synthesised inCytosolCell membraneMitochondriaNucleus 43. Most important factor which causes lactic acidosis in alcoholicsProduction of NADHFormation of acetaldehydeProduction of acetateNone of the above 44. Major metabolism of saturated fatty acids in the mitochondria is called asBeta oxidationalpha oxidationgamma oxidationnone of the above 45. In sickle cell anaemia, the defect can be explained as having arisen fromA base insertion in DNAA base deletion in DNAA base substitution in DNANone of the above 46. Metabolic water isInjested waterWater infused ivWater produced by metabolismNone 47. Fatty acids are stored asCholesterolTriglyceridesSphingomyelinnone 48. Phospholipids areSimple lipidsDerived lipidsComplex lipidsNone 49. Free fatty acid produced in adipose tissue is transported byGlobulinAlbuminCeruloplasminNone 50. True about polymerase chain reaction isEnzymatic DNA amplificationRecombinant DNA amplificationSeparation of protein fragments in serumNone 51. Differential expression of same gene depending on parent of origin is referred to asGenomic ImprintingMosaicismAnticipationNon penetrance 52. Enzymes concerned with the citric acid cycle are found in theNucleusRibosomesMitochondriaNon particulate cytoplasm 53. Glucoronide reaction is seen inPhase 1Phase 2Enzymatic reaction with NADPHNon enzymatic reaction 54. Which of the following is a true statement regarding enzymesCompetitive inhibition shows increased Km, unchanged Vmax Non competitive inhibition shows increased Km, unchanged VmaxCompetitive inhibition shows unchnaged Km,unchanged VmaxNon competitiive inhibition shows unchanged Km,unchanged Vmax 55. TTX resistant sodium channel isNAv1.1NAv1.8TVRP1NMDA 56. Binding of proteins to DNA is regulated byCopperZincSeleniumNickle 57. Vit B6 is also calledPyridoxineThiamineRiboflavinNiacin 58. The coenzyme required for the synthesis of fatty acid synthesis isFADFADH2NADNADPH 59. HMP shunt pathway producesATPADPNADPHNAD 60. Null mutation isMutation occuring in non coding regionMutation that does not change the amino acid or end productMutation that codes for a change in progeny without any chromosomal changeMutation that leads to no functional gene product 61. Same aminoacid is coded by multiple codons due to followingDegeneracyFrame-shift mutationTranscriptionMutation 62. Starting material for production of insulin from bacteria isgenomic DNA from beta pancreatic cells of humanGenomic DNA from lymphocytes of humanmRNA from beta pancreatic cells of humanmRNA from lymphocytes of human 63. Western blot is used forDNARNAProteinsmRNA 64. Which of the following is not involved in RNA interferenceDroshaPashamRNAmiRNA 65. The best source of potassium isWhole grainsRed meatDried fruitsMilk 66. Not a nucleic acid testWestern blotSouthern blotNorthern blotMicroarray 67. Which of the following techniques is used for detection of variation in DNA sequence and Gene expressionNorthern blotSouthern blotWestern blotMicroarray 68. Most abundant RNArRNAmRNAtRNAMicro RNA 69. Serotonin is derived fromArginine, Aspartate, glutamateMethionine, lysine, leucineGlycine, Arginine, glutamateTryptophan metabolism 70. For polymerase chain reaction which of the following is not requiredTAQ polymerased-NTPDideoxynucleotidesMagnesium 71. Cardiolipin is associated withNucleusMitochondriaRibosomeLysosome 72. Acetyl Co A carboxylase enzyme is present inCytosolMitochondriaBoth cytosol and mitochondriaLysosome 73. The aminoacid required for the synthesis of haem isGlutamineGlutamic acidGlycineLysine 74. Amino acid responsible for formation of histone-nucleic acid complexAlaninethreonineLeucineLysine 75. The code word of which proteins is also an initiation code for proteinsLeucineIsoleucineMethionineLysine 76. Micosatellite sequence isSmall satelliteExtra chromosomal DNAShort sequence (2-5) repeat DNALooped -DNA 77. Urea breath test is done inKetoacidosisH. pylori infectionKidney diseaseLiver disease 78. First protein to be broken down for energy in prolonged starvation is fromSkeletal muscleSmooth muscleKidneyLiver 79. Proteins in the transmembrane region areHydrophilicHydrophobicMultiple domain spanningLipophobic 80. Cholesterol is not a precursor for synthesis ofVitamin DProgesteroneBile acidLipocortin 81. Which of the following is a water soluble VitaminFolic acidVit AVit KLinolenic acid 82. Alcohol dehydrogenase is aHydrolaseOxireductaseTransferaseLigase 83. Mutation of oxidative enzymes (peroxisomes) could lead toZellweger's syndromeGaucher's diseaseEpidermolysis bullosaLeber's neuropathy 84. The predominant isozyme of LDH in cardiac muscle isLD-1LD-2LD-3LD-5 85. Most common mechanism of phagocytosis by lysosome isMPOH2O2NOlactoferrin 86. Genetic deficiencies of NADPH oxidase casueMitochondrial myopathiesChronic granulomatous diseaseDecreased ATP productionLactic acidosis 87. Which of the following is a salivary enzymeAmylaseMaltaseLipaseLactase 88. The long and short arms of chromosomes are designated respectively asp and q armsm and q armsq and p armsl and s arms 89. Sphingomyelinase deficiency is seen inNiemann-Pick diseaseFarber's diseaseTay Sach's diseaseKrabbe's disease 90. RNA "i" causes the following ina geneKnock inKnock outKnock downKnock up 91. Acetyl CoA can produce all of the following exceptGlucoseFatty acidCholesterolKetone bodies 92. one of the earliest symptoms of vit A deficiency isnyctalopiaxerophthalmiabitot spotkeratomalacia 93. Rapid method of chromosome identification in interphase isFISHPCRSSCPKaryotyping 94. Which of the following statements regarding mature cytoplasmic messenger RNA is true?Transcribed from nuclear DNAHas thiamine in place of Uracilsugar is deoxyriboseits molecular weight is more than hn-RNA 95. Maple syrup disease is due to deficiency ofBranched chain keto acid decarboxylaseHistidine deaminaseHomegentase oxidaseIsocitrate dehydrogenase deficiency 96. The two strands of DNA are held together byVan der Waal bondHydrogen bondCovalent bondIonic interactions 97. In gout, deficiency of which enzyme is seenXanthine oxidaseAMP DeaminaseHGPRTaseInosine monophosphatase 98. Statin action on HMG CoA reductase isCompetetive inhibitorIrreversible inhibitorNon competetive inhibitorInhibition result in decrease LDL receptors on hepatocytes 99. Iodine pump is stimulated byMetabolic toxinMonovalent anionConcentration of TSHInhibition of Na+-K+ pump 100. which of the following events doesnot occur when concentration of glucose in the liver decreasesinactivation of phosphofructokinase-2activation of fructose bisphosphatase-2increased levels of Fructose 2,6 bisphosphateincreased levels of glucagon Loading...